We work in human cells and also use yeast as a model system to address various questions regarding global gene regulation. We use genomic and molecular experimental methods coupled with computational analyses. The genomic methods involve extensive use of DNA microarrays and next-generation sequencing (Illumina, SOLiD).
The Xhemalçe lab aims to decipher how epigenetic modifications regulate the expression of genetic information from DNA to RNA to proteins, with a particular focus on RNA methylation pathways. We use a combination of cellular and molecular biology, RNA biochemistry, next generation sequencing and quantitative mass spectrometry to identify and characterise novel "writers", "erasers" and "readers" of RNA "epigenetic" modifications. The objective is to identify fundamental biological mechanisms of gene regulation, as well as potential therapeutic targets for cancer and metabolic diseases.
We are broadly interested in understanding evolution as a creative force. Our lab primarily uses experiments with microorganisms and molecules to watch evolution in action. We also use the tools of synthetic biology and bioengineering to manipulate and study evolutionary processes.